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- Protein purification techniques, Chapter 2-2 Buffers stabilize pH most effectively at their pK value, but can generally be used within 1 pH unit on either side. In selecting the most suitable buffer it is
- protein production and purification Strain of E. coli. For high-level protein production purposes, BL21(DE3) is an appropriate E. coli strain. It has the advantage of being deficient in both lon a